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[EP-tech] Eprints SMPT configuration Error with Office 365

CAUTION: This e-mail originated outside the University of Southampton.
Dear All,
Could anyone please help me to resolve the issue of Office365 SMTP Configuration issue with eprints.exchange.isb.edu. Let me clear what I have done so far.
2. It is working well with mail command without any isssue
3. Email.pm and SystemSettings.pm have been configured accordingly
4, Followed this thread https://www.eprints.org/eptech/msg07173.html and tested with test_sendmail.pl and working perfectly.
I am facing the following error

Connect failed :IO::Socket::INET: connect: Connection refused
 at /usr/local/share/perl/5.30.0/Net/SMTP/TLS.pm line 113.
	Net::SMTP::TLS::new("Net::SMTP::TLS", "localhost", "Hello", "localhost", "Port", 587, "User", "******email", ...) called at /opt/eprints3/perl_lib/EPrints/Email.pm line 178
	EPrints::Email::send_mail_via_smtp("sig", XML::LibXML::DocumentFragment=SCALAR(0x55c2ec290348), "to_email", "lrc_hyd\@isb.edu", "cc_list", ARRAY(0x55c2ec28fc70), "replyto_email", "****@gmail.com", ...) called at /opt/eprints3/perl_lib/EPrints/Repository.pm line 1927 
eval {...} called at /opt/eprints3/perl_lib/EPrints/Repository.pm line 1927"
 
Could anyone please help me out?

thanks
Revathi